RhoGDI Antibody (OTI1F2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73890

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RhoGDI Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoGDI Antibody (OTI1F2) - Azide and BSA Free

Western Blot: RhoGDI Antibody (OTI1F2)Azide and BSA Free [NBP2-73890]

Western Blot: RhoGDI Antibody (OTI1F2)Azide and BSA Free [NBP2-73890]

Western Blot: RhoGDI Antibody (OTI1F2) - Azide and BSA Free [NBP2-73890] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoGDI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoGDI.
Flow Cytometry: RhoGDI Antibody (OTI1F2) - Azide and BSA Free [NBP2-73890]

Flow Cytometry: RhoGDI Antibody (OTI1F2) - Azide and BSA Free [NBP2-73890]

Flow Cytometry: RhoGDI Antibody (OTI1F2) - Azide and BSA Free [NBP2-73890] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RhoGDI antibody, and then analyzed by flow cytometry.
Western Blot: RhoGDI Antibody (OTI1F2)Azide and BSA Free [NBP2-73890]

Western Blot: RhoGDI Antibody (OTI1F2)Azide and BSA Free [NBP2-73890]

Western Blot: RhoGDI Antibody (OTI1F2) - Azide and BSA Free [NBP2-73890] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RhoGDI monoclonal antibody.
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Applications for RhoGDI Antibody (OTI1F2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:200-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoGDI

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Alternate Names

GDIA1, MGC117248, Rho GDI 1, Rho GDP dissociation inhibitor (GDI) alpha, rho GDP-dissociation inhibitor 1, RHOGDI, Rho-GDI alpha, RHOGDI-1

Gene Symbol

ARHGDIA

Additional RhoGDI Products

Product Documents for RhoGDI Antibody (OTI1F2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoGDI Antibody (OTI1F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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