Rhophilin 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-792
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-TNKNRQKSAST corresponding to internal region according to NP_149094.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rhophilin 2 Antibody
![Immunohistochemistry-Paraffin: Rhophilin 2 Antibody [NB600-792]](https://resources.bio-techne.com/images/products/Rhophilin-2-Antibody-Immunohistochemistry-Paraffin-NB600-792-img0002.jpg "Immunohistochemistry-Paraffin: Rhophilin 2 Antibody [NB600-792]")
Immunohistochemistry-Paraffin: Rhophilin 2 Antibody [NB600-792]
Immunohistochemistry-Paraffin: Rhophilin 2 Antibody [NB600-792] (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Rhophilin 2 Antibody
Application
Recommended Usage
Immunohistochemistry
5 - 10 ug/ml
Immunohistochemistry-Paraffin
5 - 10 ug/ml
Peptide ELISA
Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human pancreas and prostate lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77 kDa band according to NP_149094.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rhophilin 2
Alternate Names
GTP-Rho-binding protein 2, p76RBE, RHOBP, rhophilin 2,76 kDa RhoB effector protein, rhophilin, Rho GTPase binding protein 2, rhophilin-2, rhophilin-like Rho-GTPase binding protein
Gene Symbol
RHPN2
UniProt
Additional Rhophilin 2 Products
Product Documents for Rhophilin 2 Antibody
Product Specific Notices for Rhophilin 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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