Rhot1 Antibody (4H4)
Novus Biologicals, part of Bio-Techne | Catalog # H00055288-M01
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4H4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RHOT1 (NP_060777, 483 a.a. ~ 580 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30126943). Other species not tested.
Specificity
RHOT1 - ras homolog gene family, member T1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Rhot1 Antibody (4H4)
![Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]](https://resources.bio-techne.com/images/products/Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0005.jpg "Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]")
Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]
Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - Parkin knockout improves the decline of mitochondrial dynamics proteins in SOD1-G93A spinal cord, but mitochondrial protein ubiquitination profiles and March5 and Mul1 levels are unaffected A, B Western blots (A) and quantification (B) of Miro1 in spinal cord homogenates at disease end stage. Beta-actin is used as loading reference. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888) licensed under a CC-BY license.![Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]](https://resources.bio-techne.com/images/products/Rhot1-Antibody-4H4-Western-Blot-H00055288-M01-img0004.jpg "Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]")
Western Blot: Rhot1 Antibody (4H4) [H00055288-M01]
Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - RHOT1 monoclonal antibody (M01), clone 4H4 Analysis of RHOT1 expression in Hela S3 NE.![ELISA: Rhot1 Antibody (4H4) [H00055288-M01]](https://resources.bio-techne.com/images/products/Rhot1-Antibody-4H4-ELISA-H00055288-M01-img0003.jpg "ELISA: Rhot1 Antibody (4H4) [H00055288-M01]")
ELISA: Rhot1 Antibody (4H4) [H00055288-M01]
ELISA: Rhot1 Antibody (4H4) [H00055288-M01] - Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.Applications for Rhot1 Antibody (4H4)
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Miro1/Rhot1
Long Name
Mitochondrial Rho (MIRO) GTPase 1
Alternate Names
ARHT1, Rhot1
Entrez Gene IDs
55288 (Human)
Gene Symbol
RHOT1
UniProt
Additional Miro1/Rhot1 Products
Product Documents for Rhot1 Antibody (4H4)
Product Specific Notices for Rhot1 Antibody (4H4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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