Rhot1 Antibody (CL1083)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52964

Novus Biologicals, part of Bio-Techne
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NBP2-52964
NBP2-52964-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1083

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Miro1/Rhot1 Antibodies »

Product Summary for Rhot1 Antibody (CL1083)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: THIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIE

Epitope

VTSRWIPLIN

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rhot1 Antibody (CL1083)

Western Blot: Rhot1 Antibody (CL1083) [NBP2-52964]

Western Blot: Rhot1 Antibody (CL1083) [NBP2-52964]

Western Blot: Rhot1 Antibody (CL1083) [NBP2-52964] - Lane 1: Marker [kDa], Lane 2: Human cerebral cortex
Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964]

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964]

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964] - Staining of human skeletal muscle shows weak cytoplasmic positivity in striated muscle fibers.
Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964]

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964]

Immunohistochemistry-Paraffin: Rhot1 Antibody (CL1083) [NBP2-52964] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
View 2 more images

Applications for Rhot1 Antibody (CL1083)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Miro1/Rhot1

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution

Long Name

Mitochondrial Rho (MIRO) GTPase 1

Alternate Names

ARHT1, Rhot1

Gene Symbol

RHOT1

Additional Miro1/Rhot1 Products

Product Documents for Rhot1 Antibody (CL1083)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rhot1 Antibody (CL1083)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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