RHOT2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93326

Novus Biologicals, part of Bio-Techne
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NBP2-93326-0.02ml
NBP2-93326-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RHOT2 Antibodies »

Product Summary for RHOT2 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 340-550 of human RHOT2 (NP_620124.1). PWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RHOT2 Antibody - Azide and BSA Free

Western Blot: RHOT2 AntibodyAzide and BSA Free [NBP2-93326]

Western Blot: RHOT2 AntibodyAzide and BSA Free [NBP2-93326]

Western Blot: RHOT2 Antibody [NBP2-93326] - Western blot analysis of extracts of various cell lines, using (NBP2-93326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: RHOT2 Antibody - Azide and BSA Free [NBP2-93326]

Immunohistochemistry-Paraffin: RHOT2 Antibody - Azide and BSA Free [NBP2-93326]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP2-93326] - Immunohistochemistry of paraffin-embedded rat kidney using RHOT2 Rabbit pAb (NBP2-93326) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: RHOT2 Antibody - Azide and BSA Free [NBP2-93326]

Immunohistochemistry-Paraffin: RHOT2 Antibody - Azide and BSA Free [NBP2-93326]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP2-93326] - Immunohistochemistry of paraffin-embedded mouse brain using RHOT2 Rabbit pAb (NBP2-93326) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for RHOT2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RHOT2

RHOT2 is a gene that codes for a protein that is 618 amino acids long and weighs approximately 68 kDa and has a shorter isoform that is 213 amino acids long and weighs approximately 23 kDa. The protein that RHOT2 codes for is a mitochondrial GTPase that is involved in mitochondrial trafficking as well as the control of the subcellular distribution of mitochondria. Current research is being done on diseases and disorders linked to this gene including hypoxia. RHOT2 has been shown to have interactions with ZFYVE9, RYK, TRAK1, BECN1, and CLN3 in pathways such as the Rho GTPase cycle and signal transduction pathways.

Alternate Names

ARHT2, C16orf39, chromosome 16 open reading frame 39, EC 3.6.5, EC 3.6.5.-, hMiro-2, MIRO-2mitochondrial Rho 2, mitochondrial Rho GTPase 2, Ras homolog gene family member T2, ras homolog gene family, member T2, RASL

Gene Symbol

RHOT2

Additional RHOT2 Products

Product Documents for RHOT2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RHOT2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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