Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H23

Format

BSA Free

Concentration

1.0 mg/ml

View all Ribonuclease Inhibitor Antibodies »

Product Summary for Ribonuclease Inhibitor Antibody (1H23) - BSA Free

Immunogen

Recombinant Ribonuclease inhibitor (7-461aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Ribonuclease Inhibitor Antibody (1H23) - BSA Free

Western Blot: Ribonuclease Inhibitor Antibody (1H23)BSA Free [NBP1-30167]

Western Blot: Ribonuclease Inhibitor Antibody (1H23)BSA Free [NBP1-30167]

Western Blot: Ribonuclease Inhibitor Antibody (1H23) [NBP1-30167] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RNH1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Jurkat cell lysate Lane 2. : HepG2 cell lysate
Immunocytochemistry/ Immunofluorescence: Ribonuclease Inhibitor Antibody (1H23) - BSA Free [NBP1-30167]

Immunocytochemistry/ Immunofluorescence: Ribonuclease Inhibitor Antibody (1H23) - BSA Free [NBP1-30167]

Immunocytochemistry/Immunofluorescence: Ribonuclease Inhibitor Antibody (1H23) [NBP1-30167] - Analysis of RNH1 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RNH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Ribonuclease Inhibitor Antibody (1H23) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ribonuclease Inhibitor

Ribonuclease inhibitor 1(RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.

Alternate Names

Placental ribonuclease inhibitor, Placental RNase inhibitor, PRI, RAIMGC4569, ribonuclease inhibitor, ribonuclease/angiogenin inhibitor, ribonuclease/angiogenin inhibitor 1MGC18200, RNHMGC54054

Entrez Gene IDs

6050 (Human)

Gene Symbol

RNH1

UniProt

NP_002930 (Human)

Additional Ribonuclease Inhibitor Products

Product Documents for Ribonuclease Inhibitor Antibody (1H23) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ribonuclease Inhibitor Antibody (1H23) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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