Ribophorin II Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80835

Novus Biologicals, part of Bio-Techne
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NBP1-80835
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ribophorin II Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ribophorin II Antibody

Western Blot: Ribophorin II Antibody [NBP1-80835]

Western Blot: Ribophorin II Antibody [NBP1-80835]

Western Blot: Ribophorin II Antibody [NBP1-80835] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: Ribophorin II Antibody [NBP1-80835]

Immunocytochemistry/ Immunofluorescence: Ribophorin II Antibody [NBP1-80835]

Immunocytochemistry/Immunofluorescence: Ribophorin II Antibody [NBP1-80835] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835]

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835]

Immunohistochemistry-Paraffin: Ribophorin II Antibody [NBP1-80835] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
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Applications for Ribophorin II Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ribophorin II

Ribophorin II encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.

Alternate Names

RIBIIREC 2.4.1.119, ribophorin IIDolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, Ribophorin-2, RPNII, RPN-IIdolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, SWP1

Entrez Gene IDs

6185 (Human)

Gene Symbol

RPN2

UniProt

P04844 (Human)

Additional Ribophorin II Products

Product Documents for Ribophorin II Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ribophorin II Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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