Ribosomal Protein S6/RPS6 Antibody (A6B8-R)

Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919]

Western Blot: Ribosomal Protein S6/RPS6 Antibody (A6B8-R) [NBP3-32919] - Western blot analysis of Ribosomal Protein S6/RPS6 on different lysates with Mouse anti-Ribosomal Protein S6/RPS6 antibody (NBP3-32919) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: THP-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 1 minute 22 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32919) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.