Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1553
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Ribosomal Protein S6 (GeneID 6194) around serine 235 and serine 236 according to the numbering given in entry NP_001001.2.
Modification
p Ser235, p Ser236
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
![Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553]](https://resources.bio-techne.com/images/products/Ribosomal-Protein-S6-RPS6-[p-Ser235--p-Ser236]-Antibody-Western-Blot-NB100-1553-img0002.jpg "Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553]")
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553]
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody [NB100-1553] - Detection of Human Phospho RPS6 (S235/S236) by Western Blot. Samples: Whole cell lysate (35 mcg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamcycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody NB100-1553 used at 0.04 mcg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody NB100-1595 at 0.04 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).Applications for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Western Blot
1:2500-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Ribosomal Protein S6/RPS6
Additional Ribosomal Protein S6/RPS6 Products
Product Documents for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
Product Specific Notices for Ribosomal Protein S6/RPS6 [p Ser235, p Ser236] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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