Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Rictor Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).

Specificity

NB100-611 is specific for human Rictor protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rictor Antibody

Western Blot: Rictor Antibody [NB100-611]

Western Blot: Rictor Antibody [NB100-611]

Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody NB100-611 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Rictor Antibody [NB100-611]

Immunoprecipitation: Rictor Antibody [NB100-611]

Immunoprecipitation: Rictor Antibody [NB100-611] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-611 (lot 5) used for IP at 6 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot 4) and rabbit anti-Rictor antibody NB100-612. For blotting immunoprecipitated Rictor, NB100-611 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Rictor Antibody

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Blots performed with NB 100-611 show additional bands not seen with NB 100-612.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rictor

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Long Name

Rapamycin-insensitive Companion of mTOR

Alternate Names

mAVO3, Pianissimo

Entrez Gene IDs

253260 (Human); 78757 (Mouse)

Gene Symbol

RICTOR

UniProt

Q6R327

Additional Rictor Products

Product Documents for Rictor Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rictor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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