Rictor Antibody

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions & rictor phosphorylation at Ser1235. Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin, then stimulated with 10 nM IGF-1 in presence/absence of 10 nM E2 for 24 h. Western blots for GSK3beta-pS9, GSK3beta, rictor pS1235 and rictor. At least 3 independent experiments indicate that rictor phosphorylation at S1235 by GSK-3beta downregulates mTORC2 activity. IGF-1 induced strong phosphorylation of GSK-3beta at Ser9 in the absence and presence of E2. Rapamycin pretreatment reduced phosphorylation in E2 co-treated cardiomyocytes, indicating increased activity of GSK-3beta, which was not associated with increased phosphorylation of rictor at S1235. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612]

Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - Detection of RICTOR on SKOV3 whole cell lysate, treated with PEITC. Image courtesy of product review by Parul Gupta (Texas Tech University Health Sciences Center).

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions and rictor phosphorylation at Ser1235.Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin and then stimulated with 10 nM IGF-1 in presence or absence of 10 nM E2 for 24 h. A, shown are representative western blots for mTOR, rictor and raptor; quantitative analysis was performed with mean +/- SEM of fold stimulation by IGF-1 of at least 3 independently performed experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY license.

Western Blot: Rictor Antibody [NB100-612] -

Western Blot: Rictor Antibody [NB100-612] - Truncated Sin1 displaces endogenous Sin1 from mTORC2 in DLD1 colon cancer cellsA. Schematic indicating the domain structure of Sin1 & the constructs used to displace endogenous Sin1 from mTORC2. B. Expression of myc tagged Sin1 constructs can be detected only after induction with Doxycycline (Dox). Cells were treated with 100nM of doxycycline (+) for 72 hours & expressed proteins were detected by immunoblot of whole cell lysates with anti-myc (9E10) antibodies. C. & D. Sin1 constructs incorporate into mTORC2 & displace endogenous Sin1. Constructs were induced for 72 hours prior to immune precipitation. (C) mTORC2 subunits, mTOR & Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-∆Sin1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor & mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments E. Endogenous Sin1 & Rictor immunoprecipitates demonstrate displacement of endogenous Sin1 from mTORC2. Following induction, band shifted myc-tagged FL Sin1 can be detected in Sin1 & Rictor precipitates (Left panels). Truncated ∆Sin1 can be detected in Rictor, but not Sin1, immunoprecipitates as the Sin1 antibody epitope is deleted from ∆Sin1. F. Quantification of Sin1 levels detected in Rictor immunoprecipitates indicates the level of endogenous mTORC2 disruption following Sin1 construct induction (data are mean +/- S.D; n = 3). Myc-∆Sin1 displaces >80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Rictor Antibody [NB100-612] -

Western Blot: Rictor Antibody [NB100-612] - Truncated Sin1 displaces endogenous Sin1 from mTORC2 in DLD1 colon cancer cellsA. Schematic indicating the domain structure of Sin1 & the constructs used to displace endogenous Sin1 from mTORC2. B. Expression of myc tagged Sin1 constructs can be detected only after induction with Doxycycline (Dox). Cells were treated with 100nM of doxycycline (+) for 72 hours & expressed proteins were detected by immunoblot of whole cell lysates with anti-myc (9E10) antibodies. C. & D. Sin1 constructs incorporate into mTORC2 & displace endogenous Sin1. Constructs were induced for 72 hours prior to immune precipitation. (C) mTORC2 subunits, mTOR & Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-∆Sin1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor & mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments E. Endogenous Sin1 & Rictor immunoprecipitates demonstrate displacement of endogenous Sin1 from mTORC2. Following induction, band shifted myc-tagged FL Sin1 can be detected in Sin1 & Rictor precipitates (Left panels). Truncated ∆Sin1 can be detected in Rictor, but not Sin1, immunoprecipitates as the Sin1 antibody epitope is deleted from ∆Sin1. F. Quantification of Sin1 levels detected in Rictor immunoprecipitates indicates the level of endogenous mTORC2 disruption following Sin1 construct induction (data are mean +/- S.D; n = 3). Myc-∆Sin1 displaces >80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.