Rif1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47303
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ELNVGNEASFHGQERTKTGISEEAAIEENKRNDDSEADTAKLNAKEVATEEFNSDISLSDNTTPVKLNAQTEISEQTAAGELDGGNDVSDLHSS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rif1 Antibody
Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-47303]
Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-47303] - Identification of an HDR-enhancing fragment of 53BP1. Representative IF images showing HA-tagged DN1 or DN1S and RIF1 recruitment to IR-induced DNA repair foci. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10735-7), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Rif1 Antibody [NBP2-47303]
Immunohistochemistry-Paraffin: Rif1 Antibody [NBP2-47303] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-47303]
Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-47303] - Staining human cell line HEK 293 shows positivity in nucleus. Antibody staining is shown in green.Applications for Rif1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rif1
Alternate Names
DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1
Gene Symbol
RIF1
Additional Rif1 Products
Product Documents for Rif1 Antibody
Product Specific Notices for Rif1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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