Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6C1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RIG-I Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DDX58(NP_055129) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

106.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIG-I Antibody (OTI6C1) - Azide and BSA Free

Western Blot: RIG-I Antibody (OTI6C1)Azide and BSA Free [NBP2-73909]

Western Blot: RIG-I Antibody (OTI6C1)Azide and BSA Free [NBP2-73909]

Western Blot: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIG-I.
Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909]

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909]

Immunocytochemistry/Immunofluorescence: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RIG-I.
Immunohistochemistry: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909]

Immunohistochemistry: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909]

Immunohistochemistry: RIG-I Antibody (OTI6C1) - Azide and BSA Free [NBP2-73909] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for RIG-I Antibody (OTI6C1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIG-I

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Long Name

RIG-I

Alternate Names

DDX58, RIGI

Gene Symbol

RIGI

Additional RIG-I Products

Product Documents for RIG-I Antibody (OTI6C1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RIG-I Antibody (OTI6C1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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