Skip to main content

RIOK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30103

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-30103

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

maps to a region between residue 1 and 50 of human RIO kinase 1 using the numbering given in entry NP_113668.2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIOK1 Antibody

Western Blot: RIOK1 Antibody [NBP1-30103]

Western Blot: RIOK1 Antibody [NBP1-30103]

Western Blot: RIOK1 Antibody [NBP1-30103] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RIOK1 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. RIOK1 was also immunoprecipitated by rabbit anti-RIOK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for RIOK1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RIOK1

RIO kinase 1 (RIOK1) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis.

Alternate Names

AD034, bA288G3.1, EC 2.7.11.1, FLJ30006, MGC12903, prematurely truncated due to insertion of intronic sequence, RIO kinase 1, RIO kinase 1 (yeast), RRP10, serine/threonine-protein kinase RIO1

Entrez Gene IDs

83732 (Human)

Gene Symbol

RIOK1

UniProt

Additional RIOK1 Products

Product Documents for RIOK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RIOK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...