Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RIOK2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide mapping to a region between residue 275 and 325 of human RIO kinase 2 using the numbering given in entry NP_060813.1 (GeneID 55781).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIOK2 Antibody

Western Blot: RIOK2 Antibody [NBP1-30098]

Western Blot: RIOK2 Antibody [NBP1-30098]

Western Blot: RIOK2 Antibody [NBP1-30098] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-30098 was used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RIOK2 was also immunoprecipitated by rabbit anti-RIOK2 antibody NBP1-30099, which recognizes a downstream epitope.

Applications for RIOK2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RIOK2

RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.

Long Name

Serine/threonine-protein kinase RIO2

Alternate Names

RIO kinase 2, RIO2

Entrez Gene IDs

55781 (Human)

Gene Symbol

RIOK2

UniProt

Q9BVS4 (Human)

Additional RIOK2 Products

Product Documents for RIOK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RIOK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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