RIPK1/RIP1 Antibody (2V4N8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15786

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15786-100ul
NBP3-15786-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2V4N8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RIPK1/RIP1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 289-490 of human RIPK1/RIP1 (NP_003795.2). YLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIPK1/RIP1 Antibody (2V4N8)

Western Blot: RIPK1/RIP1 Antibody (2V4N8) [NBP3-15786]

Western Blot: RIPK1/RIP1 Antibody (2V4N8) [NBP3-15786]

Western Blot: RIPK1/RIP1 Antibody (2V4N8) [NBP3-15786] - Western blot analysis of extracts of various cell lines, using RIPK1/RIP1 antibody (NBP3-15786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: RIPK1/RIP1 Antibody (2V4N8) [NBP3-15786]

Immunoprecipitation: RIPK1/RIP1 Antibody (2V4N8) [NBP3-15786]

Immunoprecipitation: RIPK1/RIP1 Antibody (2V4N8) [NBP3-15786] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RIPK1/RIP1 antibody (NBP3-15786). Western blot was performed from the immunoprecipitate using RIPK1/RIP1 antibody (NBP3-15786) at a dilition of 1:500.

Applications for RIPK1/RIP1 Antibody (2V4N8)

Application
Recommended Usage

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIPK1/RIP1

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1

Alternate Names

RIP, RIP1

Gene Symbol

RIPK1

Additional RIPK1/RIP1 Products

Product Documents for RIPK1/RIP1 Antibody (2V4N8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RIPK1/RIP1 Antibody (2V4N8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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