RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25637
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2250
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free
Immunogen
Recombinant fragment of human RIPK1/RIP1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free [NBP3-25637] -
Immunocytochemistry/Immunofluorescence: RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free [NBP3-25637] - RIP antibody [HL2250] detects RIP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RIP stained by RIP antibody [HL2250] (NBP3-25637) diluted at 1:100. Blue: Fluoroshield with DAPI .
Western Blot: RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free [NBP3-25637] -
Western Blot: RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free [NBP3-25637] - Untreated (-) and treated (+) NCI-H929 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (NBP3-25637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free [NBP3-25637] -
Western Blot: RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free [NBP3-25637] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (NBP3-25637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RIPK1/RIP1
Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1
Alternate Names
RIP, RIP1
Gene Symbol
RIPK1
Additional RIPK1/RIP1 Products
Product Documents for RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free
Product Specific Notices for RIPK1/RIP1 Antibody (HL2250) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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