RIPK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86629

Novus Biologicals, part of Bio-Techne
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NBP1-86629
NBP1-86629-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RIPK2/RIP2 Antibodies »

Product Summary for RIPK2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIPK2 Antibody

Western Blot: RIPK2 Antibody [NBP1-86629]

Western Blot: RIPK2 Antibody [NBP1-86629]

Western Blot: RIPK2 Antibody [NBP1-86629] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody [NBP1-86629]

Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody [NBP1-86629]

Immunocytochemistry/Immunofluorescence: RIPK2 Antibody [NBP1-86629] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86629]

Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86629]

Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-86629] - Staining of human placenta shows strong cytoplasmic positivity in Trophoblasts.

Applications for RIPK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIPK2/RIP2

RIPK2 encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Long Name

Receptor Interacting Serine/Threonine Kinase 2

Alternate Names

CARD3, GIG30, RICK, RIP-2, RIP2

Gene Symbol

RIPK2

Additional RIPK2/RIP2 Products

Product Documents for RIPK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RIPK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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