Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RIPK3/RIP3 Antibodies »

Product Summary for RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free

Immunogen

RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free was made to a human recombinant protein fragment corresponding to amino acids 233-518 of human RIPK3 (NP_006862) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free

Western Blot: RIPK3/RIP3 Antibody (OTI1B3)Azide and BSA Free [NBP2-73912]

Western Blot: RIPK3/RIP3 Antibody (OTI1B3)Azide and BSA Free [NBP2-73912]

Western Blot: RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free [NBP2-73912] - Analysis of RIPK3/RIP3 using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592] in HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.
Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free [NBP2-73912]

Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free [NBP2-73912]

Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free [NBP2-73912] - Analysis of Carcinoma of Human thyroid tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Western Blot: RIPK3/RIP3 Antibody (OTI1B3)Azide and BSA Free [NBP2-73912]

Western Blot: RIPK3/RIP3 Antibody (OTI1B3)Azide and BSA Free [NBP2-73912]

Western Blot: RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free [NBP2-73912] - Analysis of extracts (10ug) from 2 different cell lines using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592]. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.
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Applications for RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIPK3/RIP3

RIPK3, Receptor interacting serine/threonine kinase 3 or RIP-like protein kinase 3 (human RIPK3 isoform1 theoretical molecular weight 57kDa) is a cytosolic protein with an amino terminal active kinase domain. RIP kinases, RIPK1 and RIPK3 play a central role in the induction of necroptosis, a form of programmed and inflammatory cell death that is caspase-independent (1). Necroptosis is a form of programmed necrosis which is initiated through the activation of RIPK3 by various ligands such as Fas, LPS and TNF. Formation of the necrosome results from the interaction between RIPK1 and RIPK3 mediated through their RIP homotypic interaction motifs (RHIMs) (1, 2). Following necrosome formation, RIPK3 activation leads to the phosphorylation of mixed lineage kinase domain-like protein (MLKL) which induces its oligomerization and translocation to the cell membrane where it alters plasma membrane integrity (2). Loss of membrane integrity results in lytic cell death, release of damage associated molecular patterns (DAMPs) and inflammation (2, 3). Therefore, the interaction of RIPK3 with other RHIM proteins is necessary for the initiation of necroptosis, while the execution phase is dependent on the activation of MLKL (4).

RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).

References

1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536

2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0

3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834

4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3

Alternate Names

RIP3

Gene Symbol

RIPK3

Additional RIPK3/RIP3 Products

Product Documents for RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RIPK3/RIP3 Antibody (OTI1B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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