RIPX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89614

Novus Biologicals, part of Bio-Techne
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NBP1-89614
NBP1-89614-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RIPX Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQRLRQAERSRQSAELDNRLFKQDFGDKINSLQLEVEELTRQRNQLEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIPX Antibody

Western Blot: RIPX Antibody [NBP1-89614]

Western Blot: RIPX Antibody [NBP1-89614]

Western Blot: RIPX Antibody [NBP1-89614] - Analysis using Anti-RUFY3 antibody NBP1-89614(A) shows similar pattern to independent antibody NBP2-48558 (B).
Western Blot: RIPX Antibody [NBP1-89614]

Western Blot: RIPX Antibody [NBP1-89614]

Western Blot: RIPX Antibody [NBP1-89614] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: RIPX Antibody [NBP1-89614]

Immunocytochemistry/ Immunofluorescence: RIPX Antibody [NBP1-89614]

Immunocytochemistry/Immunofluorescence: RIPX Antibody [NBP1-89614] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining is shown in green.
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Applications for RIPX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-89614 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIPX

Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes

Alternate Names

KIAA0871SINGAR1, protein RUFY3, rap2 interacting protein x, Rap2-interacting protein x, RIPX, RUN and FYVE domain containing 3, Singar, Singar1, Single axon-regulated protein, single axon-related 1

Gene Symbol

RUFY3

Additional RIPX Products

Product Documents for RIPX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RIPX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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