Rit2 Antibody (3F2)

Novus Biologicals, part of Bio-Techne | Catalog # H00006014-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rit2 Antibodies »

Product Specifications

Immunogen

RIT2 (AAH18060, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEVENEASCSPGSASGGSREYKVVMLGAGGVGKSAMTMQFISHQFPDYHDPTIEDAYKTQVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIPLVLVGNKIDLEQFRQVSTEEGLSLAQEYNCGFFETSAALRFCIDDAFHGLVREIRKKESMPSLMEKKLKRKDCLWKKLKGSLKKKRENMT

Localization

Plasma membrane

Specificity

RIT2 - Ras-like without CAAX 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rit2 Antibody (3F2)

Sandwich ELISA: Rit2 Antibody (3F2) [H00006014-M01] - Detection limit for recombinant GST tagged RIT2 is approximately 0.3ng/ml as a capture antibody.

Applications for Rit2 Antibody (3F2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rit2

The Ras super-family comprises a group of structurally related low molecular weight (20-30 kDa) proteins, which bind GTP or GDP and exhibit a low intrinsic GTPase activity. They are involved in signal transduction and the regulation of a variety of cellular processes, including cell growth, transformation, differentiation and morphogenesis, nucleocytoplasmic transport, and apoptosis. Ras proteins promote cancer by disrupting the normal controls of cell proliferation and differentiation. All Ras subgroups (Ras, Rho, Rab, Ran, and ADP ribosylation factor) contain five highly conserved domains (G1-G5) and act as molecular switches by alternating between an active GTP-bound form and an inactive GDP-bound form. Rit2 (Ras-like protein in neurons) and Rit1 (Ras-like protein in tissues) share 50% homology to Ras. Both contain the five highly conserved domains (G1-G5), bind GTP in vitro, and are membrane-associated. However, both lack a CAAX box and their mechanism of membrane association is distinct from the typical Ras protein.

Long Name

Ras-like without CAAX 2

Alternate Names

RIBA, RIN, ROC2

Entrez Gene IDs

6014 (Human)

Gene Symbol

RIT2

UniProt

AAH18060 (Human)

Additional Rit2 Products

Product Documents for Rit2 Antibody (3F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rit2 Antibody (3F2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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