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Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2260A

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full-length Rituximab

Specificity

Detects Rituximab in Western blots in direct ELISAs. 100% cross-reactivity with Tositumomab and Ibritumomab (95% or higher sequence identity with Rituximab) and no cross-reactivity with Cetuximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Rituximab Antibody (2260A) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Rituximab

Rituximab humanised monoclonal antibody is a therapeutic reagent directed against the human CD20 cell surface antigen. Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. This antibody has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab.

Alternate Names

B lymphocyte antigen CD20, B lymphocyte surface antigen B1, Bp35, CD20, LEU 16, LEU16, Membrane spanning 4 domains subfamily A member 1, MS4A1

Additional Rituximab Products

Product Documents for Rituximab Antibody (2260A) [PE/Cy7]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rituximab Antibody (2260A) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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