RNA Helicase A Antibody (2I6C5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16435

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16435-100ul
NBP3-16435-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2I6C5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RNA Helicase A Antibodies »

Product Summary for RNA Helicase A Antibody (2I6C5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human RNA Helicase A (Q08211). ITGLRAAMEALVVEVTKQPAIISQLDPVNERMLNMIRQISRPSAAGINLMIGSTRYGDGPRPPKMARYDNGSGYRRGGSSYSGGGYGGGYSSGGYGSGGYG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Helicase A Antibody (2I6C5)

Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435]

Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435]

Western Blot: RNA Helicase A Antibody (2I6C5) [NBP3-16435] - Western blot analysis of extracts of various cell lines, using DHX9/RNA Helicase A Rabbit mAb (NBP3-16435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody (2I6C5) [NBP3-16435]

Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody (2I6C5) [NBP3-16435]

Immunocytochemistry/Immunofluorescence: RNA Helicase A Antibody (2I6C5) [NBP3-16435] - Immunofluorescence analysis of U-2 OS cells using DHX9/RNA Helicase A Rabbit mAb (NBP3-16435) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody (2I6C5) [NBP3-16435]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody (2I6C5) [NBP3-16435]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody (2I6C5) [NBP3-16435] - Immunohistochemistry of paraffin-embedded mouse spinal cord using DHX9/RNA Helicase A Rabbit mAb (NBP3-16435) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for RNA Helicase A Antibody (2I6C5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:1000 - 1:5000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNA Helicase A

DHX9, also known as ATP-dependent RNA helicase A, consists of a 141 kDa and a 24.3 kDa isoform and is involved in activating transcription and unwinding DNA and RNA helixes in the 3' to 5' direction. Current research is being conducted on diseases and disorders such as influenza, hepatitis, t-cell leukemia, astigmatism, keratitis, immunodeficiency, Werner syndrome, and arthritis. The protein interacts in mRNA splicing, mRNA processing, and gene expression pathways with HIST1H3A- HIST13E proteins.

Long Name

ATP-dependent RNA helicase A

Alternate Names

DDX9, DEAH box protein 9, DExH-box helicase 9, DHX9, EC 3.6.4.13, Leukophysin, LKP, NDH II, NDH2

Gene Symbol

DHX9

Additional RNA Helicase A Products

Product Documents for RNA Helicase A Antibody (2I6C5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RNA Helicase A Antibody (2I6C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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