RNA polymerase beta Antibody (JE32-42)

Western Blot: RNA polymerase beta Antibody (JE32-42) [NBP3-32909]

Western Blot: RNA polymerase beta Antibody (JE32-42) [NBP3-32909] - Western blot analysis of RNA polymerase beta on different lysates with Rabbit anti-RNA polymerase beta antibody (NBP3-32909) at 1/2,000 dilution.

Lane 1: E.coli cell lysate
Lane 2: E.coli cell lysate (no heat)

Lysates/proteins at 10 ug/Lane.

Predicted band size: 150 kDa
Observed band size: 150 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32909) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.