Key Product Details

Species Reactivity

Human, Yeast

Applications

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CTD 4H8

Format

BSA Free

Concentration

1 mg/ml

View all RNA Polymerase II/POLR2A Antibodies »

Product Specifications

Immunogen

Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Reactivity Notes

Saccharomyces cerevisiae

Modification

p Ser5

Specificity

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) - BSA Free

Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8)BSA Free [NBP2-50274]

Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8)BSA Free [NBP2-50274]

Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.

Applications for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): HeLa cell line. This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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