RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49546R

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-49546AF350

Alexa Fluor 405

NBP1-49546AF405

Alexa Fluor 488

NBP1-49546AF488

Alexa Fluor 532

NBP1-49546AF532

Alexa Fluor 594

NBP1-49546AF594

Alexa Fluor 647

NBP1-49546AF647

Alexa Fluor 700

NBP1-49546AF700

Alexa Fluor 750

NBP1-49546AF750

Allophycocyanin

NBP1-49546APC

Biotin

NBP1-49546B

CoraFluor 1

NBP1-49546CL1

DyLight 350

NBP1-49546UV

DyLight 405

NBP1-49546V

DyLight 488

NBP1-49546G

DyLight 594

NBP1-49546DL594

DyLight 650

NBP1-49546C

DyLight 680

NBP1-49546FR

DyLight 755

NBP1-49546IR

HRP

NBP1-49546H

Janelia Fluor 525

NBP1-49546JF525

Janelia Fluor 549

NBP1-49546JF549

Janelia Fluor 585

NBP1-49546JF585

Janelia Fluor 635

NBP1-49546JF635

Janelia Fluor 646

NBP1-49546JF646

Janelia Fluor 669

NBP1-49546JF669

mFluor Violet 450 SE

NBP1-49546MFV450

mFluor Violet 500 SE

NBP1-49546MFV500

mFluor Violet 610 SE

NBP1-49546MFV610

PE

NBP1-49546PE

PerCP

NBP1-49546PCP

Forumulation

Catalog #

BSA Free

NBP1-49546

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Chicken, Drosophila, Yeast

Applications

Dot Blot, Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RNA Polymerase II/POLR2A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]

Modification

p Thr4

Localization

Nucleus.

Specificity

This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 550]

Application

Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RNA Polymerase II/POLR2A

RNA polymerase II catalyzes DNA transcription into RNA, and is composed of 12 subunits. The largest of these subunits is B1 (RPB1, POLR2A), which contains the DNA binding domain and the enzymatic subunit. RPB1 contains multiple heptapeptide repeats in the CTD arranged in the sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 (YSPTSPS). Modifications of this sequence, such as phosphorylation, alter polymerase activity. Serines 2, 5, and 7 are known to become phosphorylated to lead the complex through the transcription cycle, as well as functions in polyadenylation, mRNA capping, and snRNA-processing.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 550]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for RNA Polymerase II/POLR2A [p Thr4] Antibody [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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