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RNA Polymerase II/POLR2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87785

Novus Biologicals, part of Bio-Techne
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NBP1-87785
NBP1-87785-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A Antibody

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP1-87785]

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP1-87785]

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody [NBP1-87785] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A Antibody [NBP1-87785]

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A Antibody [NBP1-87785]

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A Antibody [NBP1-87785] - Staining of human testis shows nuclear positivity in cells in seminiferous ducts.

Applications for RNA Polymerase II/POLR2A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNA Polymerase II/POLR2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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