RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16906
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A phospho specific peptide corresponding to residues surrounding S7 of human RNA Polymerase II/POLR2A. SPSYS
Modification
p Ser 7
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free
![Western Blot: RNA Polymerase II/POLR2A [p Ser7] AntibodyAzide and BSA Free [NBP3-16906]](https://resources.bio-techne.com/images/products/RNA-Polymerase-II-POLR2A-[p-Ser7]-Antibody-Western-Blot-NBP3-16906-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser7] AntibodyAzide and BSA Free [NBP3-16906]")
Western Blot: RNA Polymerase II/POLR2A [p Ser7] AntibodyAzide and BSA Free [NBP3-16906]
Western Blot: RNA Polymerase II/POLR2A [p Ser7] Antibody [NBP3-16906] - Western blot analysis of extracts of MCF7 cells, using RNA Polymerase II/POLR2A antibody (NBP3-16906) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Applications for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RNA Polymerase II/POLR2A
Long Name
DNA-directed RNA Polymerase II Subunit RPB1
Alternate Names
POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2
Gene Symbol
POLR2A
Additional RNA Polymerase II/POLR2A Products
Product Documents for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free
Product Specific Notices for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov