RNase H1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20171
Key Product Details
Species Reactivity
Validated:
Human, Zebrafish
Cited:
Human, Mouse
Predicted:
Canine (94%), Chimpanzee (100%), Rabbit (91%), Rhesus Macaque (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human RNase H1. The exact sequence is proprietary.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:34937926). Bovine (87%), Guinea pig (87%), Pig (87%), Sheep (87%), Rat (89%). .
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RNase H1 Antibody
![Western Blot: RNase H1 Antibody [NBP2-20171]](https://resources.bio-techne.com/images/products/RNase-H1-Antibody-Western-Blot-NBP2-20171-img0005.jpg "Western Blot: RNase H1 Antibody [NBP2-20171]")
Western Blot: RNase H1 Antibody [NBP2-20171]
Western Blot: RNase H1 Antibody [NBP2-20171] - Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] (NBP2-20171) diluted at 1:1000.![Immunohistochemistry-Paraffin: RNase H1 Antibody [NBP2-20171]](https://resources.bio-techne.com/images/products/RNase-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-20171-img0002.jpg "Immunohistochemistry-Paraffin: RNase H1 Antibody [NBP2-20171]")
Immunohistochemistry-Paraffin: RNase H1 Antibody [NBP2-20171]
Immunohistochemistry-Paraffin: RNase H1 Antibody [NBP2-20171] - Human breast cancer, using RNase H1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for RNase H1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
Reported in scientific literature (PMID: 26106861)
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNase H1
Alternate Names
EC 3.1.26.4, H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNH1
Gene Symbol
RNASEH1
UniProt
Additional RNase H1 Products
Product Documents for RNase H1 Antibody
Product Specific Notices for RNase H1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.