RNase L Antibody (2E9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-351

Novus Biologicals, part of Bio-Techne
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NB100-351
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Hamster, Mouse (Negative), Primate, Rat (Negative)

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E9

Format

BSA Free

Concentration

1.0 mg/ml

View all RNase L Antibodies »

Product Specifications

Immunogen

Purified full-length recombinant human RNaseL produced in insect cells. [UniProt# Q05823]

Reactivity Notes

This antibody does not react with mouse or rat.

Localization

Cytoplasmic and Mitochondrial.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNase L Antibody (2E9) - BSA Free

Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9) - BSA Free [NB100-351] - Analysis for RNase L in WT and eIF2a-S51A MEF cell lines transduced with RNase L-encoding lentivirus. n.s. indicates nonspecific band for the purpose of showing equal loading. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31896577/) licensed under a CC-BY license.
Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]

Immunohistochemistry: RNase L Antibody (2E9) - BSA Free [NB100-351]

Immunohistochemistry: RNase L Antibody (2E9) [NB100-351] - RNase L antibody was tested in human colon using DAB with hematoxylin counterstain.
Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9)BSA Free [NB100-351]

Western Blot: RNase L Antibody (2E9) [NB100-351] - RNaseL in insect cell extracts transfected with recombinant human RNase L (0.1 ug) and Hey1B (100 ug)
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Applications for RNase L Antibody (2E9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:1000-1:4000 (ECL)
Application Notes
This RNase L (2E9) antibody is useful for ELISA, Western Blot and Immunohistochemistry on paraffin-embedded sections. By WB, this antibody recognizes a band at ~83 kDa, representing RNase L. A low-molecular mass, 37 kDa, isoform of RNase L has been described in peripheral blood mononuclear cell extracts. In IHC-P, staining was observed in the cytoplasm of human colon cells. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNase L

A virally induced endoribonuclease, RNase L is the terminal factor of the anti-viral action of interferon. Activation of RNase L results in inhibition of viral proliferation. Activation of RNase L has also been determined to degrade RNA, thus, initiating a cellular stress response, inducing apoptosis. Since mutations in RNase L relate to an increased incidence of prostate cancer, the activation of RNase L functions in counteracting prostate cancer, via apoptosis.

Alternate Names

2-5A-dependent ribonuclease, 2-5A-dependent RNase, 2'-5'-oligoisoadenylate synthetase-dependent, EC 3.1.26.-, HPC1, interferon-induced 2-5A-dependent RNase, MGC104972, MGC133329, PRCA1, prostate cancer 1, Ribonuclease 4, Ribonuclease L, ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent), RNase L, RNS4DKFZp781D08126

Gene Symbol

RNASEL

Additional RNase L Products

Product Documents for RNase L Antibody (2E9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNase L Antibody (2E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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