RNASEH2A Antibody (3G5-F5)
Novus Biologicals, part of Bio-Techne | Catalog # H00010535-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3G5-F5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL
Reactivity Notes
Human. Other species not tested.
Localization
Nuclear
Specificity
RNASEH2A - ribonuclease H2, large subunit
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for RNASEH2A Antibody (3G5-F5)
![Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]](https://resources.bio-techne.com/images/products/RNASEH2A-Antibody-3G5-F5-Western-Blot-H00010535-M01-img0006.jpg "Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]")
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Analysis of RNASEH2A expression in Jurkat.![Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]](https://resources.bio-techne.com/images/products/RNASEH2A-Antibody-3G5-F5-Western-Blot-H00010535-M01-img0004.jpg "Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]")
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # H00010535-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]](https://resources.bio-techne.com/images/products/RNASEH2A-Antibody-3G5-F5-Western-Blot-H00010535-M01-img0007.jpg "Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]")
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.Applications for RNASEH2A Antibody (3G5-F5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNASEH2A
Alternate Names
AGS4RNase H2 subunit A, Aicardi-Goutieres syndrome 4, Aicardi-Goutieres syndrome 4 protein, JUNB, ribonuclease H2 subunit A, ribonuclease H2, large subunit, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, ribonuclease HI, large subunit, RNase H(35), RNASEHIEC 3.1.26.4, RNHIARNase HI large subunit, RNHL
Entrez Gene IDs
10535 (Human)
Gene Symbol
RNASEH2A
OMIM
606034 (Human)
UniProt
Additional RNASEH2A Products
Product Documents for RNASEH2A Antibody (3G5-F5)
Product Specific Notices for RNASEH2A Antibody (3G5-F5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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