Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RNASEH2A Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 250 to 300 of human Ribonuclease H2, Subunit A using the numbering given in entry NP_006388.2 (GeneID 10535).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNASEH2A Antibody

Western Blot: RNASEH2A Antibody [NBP2-32144]

Western Blot: RNASEH2A Antibody [NBP2-32144]

Western Blot: RNASEH2A Antibody [NBP2-32144] - Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody NBP2-32144 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: RNASEH2A Antibody [NBP2-32144]

Immunoprecipitation: RNASEH2A Antibody [NBP2-32144]

Immunoprecipitation: RNASEH2A Antibody [NBP2-32144] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody NBP2-32144 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RNASEH2A, NBP2-32144 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RNASEH2A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RNASEH2A

Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.

Alternate Names

AGS4RNase H2 subunit A, Aicardi-Goutieres syndrome 4, Aicardi-Goutieres syndrome 4 protein, JUNB, ribonuclease H2 subunit A, ribonuclease H2, large subunit, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, ribonuclease HI, large subunit, RNase H(35), RNASEHIEC 3.1.26.4, RNHIARNase HI large subunit, RNHL

Gene Symbol

RNASEH2A

UniProt

NP_006388.2 (Human)

Additional RNASEH2A Products

Product Documents for RNASEH2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNASEH2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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