RNF114 Antibody (PSH01-99)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32910
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-99
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human RNF114 aa 1-228 / 228. (Uniprot: Q9Y508)
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RNF114 Antibody (PSH01-99)
Western Blot: RNF114 Antibody (PSH01-99) [NBP3-32910]
Western Blot: RNF114 Antibody (PSH01-99) [NBP3-32910] - Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (NBP3-32910) at 1/1,000 dilution.Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 40 ug/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32910) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for RNF114 Antibody (PSH01-99)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF114
Long Name
Ring Finger Protein 114
Alternate Names
PSORS12, ZNF228, ZNF313
Gene Symbol
RNF114
Additional RNF114 Products
Product Documents for RNF114 Antibody (PSH01-99)
Product Specific Notices for RNF114 Antibody (PSH01-99)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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