RNF114 Antibody (PSH01-99)

Western Blot: RNF114 Antibody (PSH01-99) [NBP3-32910]

Western Blot: RNF114 Antibody (PSH01-99) [NBP3-32910] - Western blot analysis of RNF114 on different lysates with Rabbit anti-RNF114 antibody (NBP3-32910) at 1/1,000 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32910) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.