RNF139 Antibody (3D10)

Novus Biologicals, part of Bio-Techne | Catalog # H00011236-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNF139 Antibodies »

Product Specifications

Immunogen

RNF139 (NP_009149, 565 a.a. ~ 664 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD

Reactivity Notes

Human. Other species not tested.

Specificity

RNF139 - ring finger protein 139

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RNF139 Antibody (3D10)

Western Blot: RNF139 Antibody (3D10) [H00011236-M01]

Western Blot: RNF139 Antibody (3D10) [H00011236-M01]

Western Blot: RNF139 Antibody (3D10) [H00011236-M01] - RNF139 monoclonal antibody (M01), clone 3D10 Analysis of RNF139 expression in HepG2.
Immunohistochemistry-Paraffin: RNF139 Antibody (3D10) [H00011236-M01]

Immunohistochemistry-Paraffin: RNF139 Antibody (3D10) [H00011236-M01]

Immunohistochemistry-Paraffin: RNF139 Antibody (3D10) [H00011236-M01] - Analysis of monoclonal antibody to RNF139 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Sandwich ELISA: RNF139 Antibody (3D10) [H00011236-M01] - Detection limit for recombinant GST tagged RNF139 is approximately 0.03ng/ml as a capture antibody.

Applications for RNF139 Antibody (3D10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF139

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Alternate Names

EC 6.3.2.-, HRCA1, patched related protein translocated in renal cancer, RCA1multiple membrane spanning receptor TRC8, ring finger protein 139MGC31961, Translocation in renal carcinoma on chromosome 8 protein, TRC8E3 ubiquitin-protein ligase RNF139

Entrez Gene IDs

11236 (Human)

Gene Symbol

RNF139

OMIM

144700 (Human)

UniProt

NP_009149 (Human)

Additional RNF139 Products

Product Documents for RNF139 Antibody (3D10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF139 Antibody (3D10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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