RNF139 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-23027

Novus Biologicals, part of Bio-Techne
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NBP1-23027
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RNF139 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HTGAAAEEFNDDT corresponding to C-Terminus according to NP_009149.2.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RNF139 Antibody

Western Blot: RNF139 Antibody [NBP1-23027]

Western Blot: RNF139 Antibody [NBP1-23027]

Western Blot: RNF139 Antibody [NBP1-23027] - (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-23027]

Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-23027]

Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-23027] - (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RNF139 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 4 ug/ml

Immunohistochemistry-Paraffin

3 - 4 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human skeletal muscle lysates (calculated MW of 76.0 kDa band according to NP_009149.2). IHC-P: Human placenta shows membranous staining in the cytotrophoblasts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF139

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Alternate Names

EC 6.3.2.-, HRCA1, patched related protein translocated in renal cancer, RCA1multiple membrane spanning receptor TRC8, ring finger protein 139MGC31961, Translocation in renal carcinoma on chromosome 8 protein, TRC8E3 ubiquitin-protein ligase RNF139

Entrez Gene IDs

11236 (Human)

Gene Symbol

RNF139

UniProt

NP_009149.2 (Human)

Additional RNF139 Products

Product Documents for RNF139 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RNF139 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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