RNF141 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80603

Novus Biologicals, part of Bio-Techne
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NBP1-80603
NBP1-80603-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (99%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNF141 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLVINKLPEKVAKHVTLVRESGSLTYEEFLGRVAELNDVTAKVASGQEKHLLFEVQPGSDSSAFWKVVVRVVCTKINKSSGIVEASRIMNLYQFIQLYKDITSQAAGVLAQSSTSEEPDENSSSVTSCQASLWMGRVKQLTDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF141 Antibody

Western Blot: RNF141 Antibody [NBP1-80603]

Western Blot: RNF141 Antibody [NBP1-80603]

Western Blot: RNF141 Antibody [NBP1-80603] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RNF141 Antibody [NBP1-80603]

Immunocytochemistry/ Immunofluorescence: RNF141 Antibody [NBP1-80603]

Immunocytochemistry/Immunofluorescence: RNF141 Antibody [NBP1-80603] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.
Immunohistochemistry-Paraffin: RNF141 Antibody [NBP1-80603]

Immunohistochemistry-Paraffin: RNF141 Antibody [NBP1-80603]

Immunohistochemistry-Paraffin: RNF141 Antibody [NBP1-80603] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.

Applications for RNF141 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF141

RNF141 is encoded by this gene contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. Abundant expression of this gene was found in the testicular tissue of fertile men, but was not detected in azoospermic patients. Studies of the mouse counterpart suggest that this gene may function as a testis specific transcription factor during spermatogenesis.

Alternate Names

C3HC4-like zinc finger protein, MGC8715, ring finger protein 141, Zinc finger protein 230, ZNF230ZFP26

Entrez Gene IDs

50862 (Human)

Gene Symbol

RNF141

Additional RNF141 Products

Product Documents for RNF141 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF141 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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