RNF149 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83440

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-83440
NBP1-83440-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNF149 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLLHTVKHGEKGIDVDAENCAVCIENFKVKDIIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPDDDGSDESSPPSASPAESEPQCDPSFKGDAGENT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF149 Antibody

Western Blot: RNF149 Antibody [NBP1-83440]

Western Blot: RNF149 Antibody [NBP1-83440]

Western Blot: RNF149 Antibody [NBP1-83440] - Analysis in control (vector only transfected HEK293T lysate) and RNF149 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RNF149 Antibody [NBP1-83440]

Immunocytochemistry/ Immunofluorescence: RNF149 Antibody [NBP1-83440]

Immunocytochemistry/Immunofluorescence: RNF149 Antibody [NBP1-83440] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440]

Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440]

Immunohistochemistry-Paraffin: RNF149 Antibody [NBP1-83440] - Staining of human small intestine shows distinct membranous positivity in glandular cells.

Applications for RNF149 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF149

Alternate Names

DNA polymerase-transactivated protein 2, DNAPTP2, E3 ubiquitin-protein ligase RNF149, EC 6.3.2.-, ring finger protein 149FLJ90504

Gene Symbol

RNF149

Additional RNF149 Products

Product Documents for RNF149 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RNF149 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.