RNF150 Antibody (5D11)
Novus Biologicals, part of Bio-Techne | Catalog # H00057484-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5D11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNF150 (NP_065775, 67 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LHTEKTECGRYGEHSPKQDARGEVVMASSAHDRLACDPNTKFAAPTRGKNWIALIPKGNCTYRDKIRNAFLQNASAVVIFNVGSNTNETITMPHAGVEDIVAIMIPEPKG
Reactivity Notes
Human. Other species not tested.
Specificity
RNF150 - ring finger protein 150
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for RNF150 Antibody (5D11)
![Western Blot: RNF150 Antibody (5D11) [H00057484-M01]](https://resources.bio-techne.com/images/products/RNF150-Antibody-5D11-Western-Blot-H00057484-M01-img0002.jpg "Western Blot: RNF150 Antibody (5D11) [H00057484-M01]")
Western Blot: RNF150 Antibody (5D11) [H00057484-M01]
Western Blot: RNF150 Antibody (5D11) [H00057484-M01] - Analysis of RNF150 expression in transfected 293T cell line by RNF150 monoclonal antibody (M01), clone 5D11.Lane 1: RNF150 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: RNF150 Antibody (5D11) [H00057484-M01] - Detection limit for recombinant GST tagged RNF150 is 1 ng/ml as a capture antibody.
Applications for RNF150 Antibody (5D11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNF150
Alternate Names
KIAA1214, MGC125502, ring finger protein 150
Entrez Gene IDs
57484 (Human)
Gene Symbol
RNF150
UniProt
Additional RNF150 Products
Product Documents for RNF150 Antibody (5D11)
Product Specific Notices for RNF150 Antibody (5D11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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