RNF181 Antibody (5A7)
Novus Biologicals, part of Bio-Techne | Catalog # H00051255-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5A7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNF181 (NP_057578, 90 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IEMPCHHLFHSSCILPWLSKTNSCPLCRYELPTDDDTYEEHRRDKARKQQQQHRLENLHGAMYT
Reactivity Notes
Human. Other species not tested.
Specificity
LOC51255 - hypothetical protein LOC51255
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for RNF181 Antibody (5A7)
![Western Blot: RNF181 Antibody (5A7) [H00051255-M01]](https://resources.bio-techne.com/images/products/RNF181-Antibody-5A7-Western-Blot-H00051255-M01-img0006.jpg "Western Blot: RNF181 Antibody (5A7) [H00051255-M01]")
Western Blot: RNF181 Antibody (5A7) [H00051255-M01]
Western Blot: RNF181 Antibody (5A7) [H00051255-M01] - RNF181 monoclonal antibody (M01), clone 5A7 Analysis of RNF181 expression in Jurkat.![Western Blot: RNF181 Antibody (5A7) [H00051255-M01]](https://resources.bio-techne.com/images/products/RNF181-Antibody-5A7-Western-Blot-H00051255-M01-img0007.jpg "Western Blot: RNF181 Antibody (5A7) [H00051255-M01]")
Western Blot: RNF181 Antibody (5A7) [H00051255-M01]
Western Blot: RNF181 Antibody (5A7) [H00051255-M01] - Analysis of RNF181 expression in transfected 293T cell line by RNF181 monoclonal antibody (M01), clone 5A7.Lane 1: RNF181 transfected lysate(17.9 KDa).Lane 2: Non-transfected lysate.![Immunoprecipitation: RNF181 Antibody (5A7) [H00051255-M01]](https://resources.bio-techne.com/images/products/RNF181-Antibody-5A7-Immunoprecipitation-H00051255-M01-img0004.jpg "Immunoprecipitation: RNF181 Antibody (5A7) [H00051255-M01]")
Immunoprecipitation: RNF181 Antibody (5A7) [H00051255-M01]
Immunoprecipitation: RNF181 Antibody (5A7) [H00051255-M01] - Analysis of RNF181 transfected lysate using anti-RNF181 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF181 monoclonal antibody.Applications for RNF181 Antibody (5A7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNF181
Alternate Names
E3 ubiquitin-protein ligase RNF181, EC 6.3.2.-, ring finger protein 181HSPC238
Entrez Gene IDs
51255 (Human)
Gene Symbol
RNF181
UniProt
Additional RNF181 Products
Product Documents for RNF181 Antibody (5A7)
Product Specific Notices for RNF181 Antibody (5A7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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