RNF2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49966
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation Sequencing, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 150 and 200 of human Ring Finger Protein 2 using the numbering given in entry NP_009143.1 (GeneID 6045).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF2 Antibody
Western Blot: RNF2 Antibody [NBP1-49966]
Western Blot: RNF2 Antibody [NBP1-49966] - Detection of Human and Mouse RNF2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from K562 (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNF2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNF2 was also immunoprecipitated by rabbit anti-RNF2 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated RNF2 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966]
Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-49966] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector ImmPACT NovaREDImmunohistochemistry: RNF2 Antibody [NBP1-49966]
Immunohistochemistry: RNF2 Antibody [NBP1-49966] - Epigenetic remodelers were overexpressed in pancreatitis and pancreatic cancer. Immunohistochemistry staining against Bmi1, Ring1b (RNF2) and H2AK119ub of pancreatic tissue from three week old KPC mice representing morphological normal tissue, from ten week old mice showing preinvasive ductal lesions and from 20 week old KPC mice demonstrating full PDAC (n = 5). High magnification images represent nuclear staining of centroacinar cells (3w-normal) or ADMs (10w-ADM). Bar, 100 um. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/fulltext/6717), licensed under a CC-BY license.Applications for RNF2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RNF2
Long Name
Ring Finger Protein 2
Alternate Names
DING, HIPI3, RING1B, RING2
Gene Symbol
RNF2
UniProt
Additional RNF2 Products
Product Documents for RNF2 Antibody
Product Specific Notices for RNF2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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