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RNF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88712

Novus Biologicals, part of Bio-Techne
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NBP1-88712
NBP1-88712-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for RNF2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF2 Antibody

Western Blot: RNF2 Antibody [NBP1-88712]

Western Blot: RNF2 Antibody [NBP1-88712]

Western Blot: RNF2 Antibody [NBP1-88712] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: RNF2 Antibody [NBP1-88712]

Immunocytochemistry/ Immunofluorescence: RNF2 Antibody [NBP1-88712]

Immunocytochemistry/Immunofluorescence: RNF2 Antibody [NBP1-88712] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-88712]

Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-88712]

Immunohistochemistry-Paraffin: RNF2 Antibody [NBP1-88712] - Staining of human urinary bladder shows nuclear positivity in urothelial cells.

Applications for RNF2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF2

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Long Name

Ring Finger Protein 2

Alternate Names

DING, HIPI3, RING1B, RING2

Gene Symbol

RNF2

Additional RNF2 Products

Product Documents for RNF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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