RNF220 Antibody (5E5)
Novus Biologicals, part of Bio-Techne | Catalog # H00055182-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5E5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
FLJ10597 (NP_060620, 468 a.a. ~ 566 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL
Reactivity Notes
Human. Other species not tested.
Specificity
C1orf164 - hypothetical protein FLJ10597
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for RNF220 Antibody (5E5)
![Immunocytochemistry/ Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01]](https://resources.bio-techne.com/images/products/RNF220-Antibody-5E5-Immunocytochemistry-Immunofluorescence-H00055182-M01-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01]")
Immunocytochemistry/ Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01]
Immunocytochemistry/Immunofluorescence: RNF220 Antibody (5E5) [H00055182-M01] - Analysis of monoclonal antibody to RNF220 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: RNF220 Antibody (5E5) [H00055182-M01] - Detection limit for recombinant GST tagged C1orf164 is approximately 0.03ng/ml as a capture antibody.
Applications for RNF220 Antibody (5E5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein on ELISA. GST alone used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNF220
Alternate Names
C1orf164, chromosome 1 open reading frame 164, E3 ubiquitin-protein ligase RNF220, EC 6.3.2.-, ring finger protein 220FLJ10597
Entrez Gene IDs
55182 (Human)
Gene Symbol
RNF220
UniProt
Additional RNF220 Products
Product Documents for RNF220 Antibody (5E5)
Product Specific Notices for RNF220 Antibody (5E5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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