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RNF23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1187

Novus Biologicals, part of Bio-Techne
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NB100-1187

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-NAAPLTIRPPTDWE corresponding to C-Terminus according to NP_067076.2, NP_742013.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_067076.2 and NP_742013.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RNF23 Antibody

Immunohistochemistry-Paraffin: RNF23 Antibody [NB100-1187]

Immunohistochemistry-Paraffin: RNF23 Antibody [NB100-1187]

Immunohistochemistry-Paraffin: RNF23 Antibody [NB100-1187] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RNF23 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 49 kDa band in human testis and rat testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4 kDa band according to NP_NP_742013.1 and of 59.7 kDa band according to NP_067076.2. The 49 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Uterus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF23

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. This gene lies within the major histocompatibility complex class I region on chromosome 6. Alternate splicing results in two transcript variants encoding different isoforms.

Alternate Names

MGC32984, RING finger protein 23RNF23TFPtripartite motif-containing 39, Testis-abundant finger protein, TRIM39B, tripartite motif containing 39, tripartite motif-containing protein 39

Entrez Gene IDs

56658 (Human); 79263 (Mouse)

Gene Symbol

TRIM39

Additional RNF23 Products

Product Documents for RNF23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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