RNF34 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77903

Novus Biologicals, part of Bio-Techne
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NBP1-77903
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Orangutan (99%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all RNF34 Antibodies »

Product Specifications

Immunogen

This affinity purified RNF34 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein. (Uniprot: Q969K3)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence. Expect reactivity with isoform 1 and 2 of RNF34. Reactivity against homologues from other sources is not known.

Specificity

This protein A purified antibody is directed against human RNF34 protein. A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence. Expect reactivity with isoform 1 and 2 of RNF34. Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This protein A purified antibody is directed against human RNF34 protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for RNF34 Antibody

Western Blot: RNF34 Antibody [NBP1-77903]

Western Blot: RNF34 Antibody [NBP1-77903]

Western Blot: RNF34 Antibody [NBP1-77903] - Shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 ul of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.

Applications for RNF34 Antibody

Application
Recommended Usage

ELISA

1:4000-1:20000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500-1:3000
Application Notes
This protein A purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract. Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 500 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: RNF34

RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation. RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RNF34 protects cells against apoptosis induced by TNF and may bind phosphatidylinositol phosphates. RNF34 is localized to both the nucleus and the cytoplasm. In the nucleus, a distinct speckled pattern is observed by immunofluorescence microscopy. In the cytoplasm, the protein is associated with membranes. RNF34 is expressed ubiquitously and can be detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Multiple transcript variants have been reported for this protein.

Alternate Names

CARP1, Caspase regulator CARP1, Caspases-8 and -10-associated RING finger protein 1, E3 ubiquitin-protein ligase RNF34, EC 6.3.2, EC 6.3.2.-, FLJ21786, FYVE-RING finger protein Momo, hRFI, Human RING finger homologous to inhibitor of apoptosis protein, RFI, RIF, RIFF, ring finger protein 34CARP-1, RING finger protein RIFF

Gene Symbol

RNF34

UniProt

Q969K3 (Human), NP_919247 (Human)

Additional RNF34 Products

Product Documents for RNF34 Antibody

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Certificate of Analysis

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Product Specific Notices for RNF34 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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