RNF36 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1207

Novus Biologicals, part of Bio-Techne
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NB100-1207
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RNF36 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GENKEPLHILHPQ corresponding to C-Terminus according to NP_892030.3, NP_542783.2.

Epitope

C-GENKEPLHILHPQ

Specificity

This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RNF36 Antibody

Western Blot: RNF36 Antibody [NB100-1207]

Western Blot: RNF36 Antibody [NB100-1207]

Western Blot: RNF36 Antibody [NB100-1207] - (0.5ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - Staining of paraffin embedded Human Cerebellum at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207]

Immunohistochemistry-Paraffin: RNF36 Antibody [NB100-1207] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RNF36 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in mouse testis lysates (calculated MW of 57.3 kDa band according to mouse NP_536771.2). IHC-P: Human testis shows speckled staining in nuclei of spermatocytes and spermitids.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF36

The gene encoding this protein is specifically expressed in germ cells at round spermatid stages during spermatogenesis. The protein encoded by this gene contains an N-terminal RING finger motif, a B-box, and a C-terminal B-30.2-like domain. When overexpressed in COS-7 and HEK-293 cells, this protein was shown to induce cell apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

RFP-like domain-containing protein trimless, RING finger protein 36HSD34, RNF36, Trif, TRIMLESS, tripartite motif containing 69, tripartite motif protein 69, tripartite motif-containing 69, tripartite motif-containing protein 69

Entrez Gene IDs

140691 (Human)

Gene Symbol

TRIM69

UniProt

NP_892030.3 (Human)

Additional RNF36 Products

Product Documents for RNF36 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF36 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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