RNF5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04014
![Immunoprecipitation: RNF5 Antibody [NBP2-04014]](https://resources.bio-techne.com/images/products/RNF5-Antibody-Immunoprecipitation-NBP2-04014-img0001.jpg "Immunoprecipitation: RNF5 Antibody [NBP2-04014]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger Protein 5 using the numbering given in entry NP_008844.1 (GeneID 6048).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF5 Antibody - BSA Free
Immunoprecipitation: RNF5 Antibody [NBP2-04014]
Immunoprecipitation: RNF5 Antibody [NBP2-04014] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF5 antibody NBP2-04014 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RNF5 was also immunoprecipitated by rabbit anti-RNF5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Western Blot: RNF5 Antibody [NBP2-04014] -
Western Blot: RNF5 Antibody [NBP2-04014] - Whole cell lysate (50 µg) from K-562, Hep-G2, RKO,LNCaP, and HeLa cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-RNF5 antibody used for WB at 0.4 µg/ml. Detection:Chemiluminescence with an exposure time of 30 secondsApplications for RNF5 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
6 µg/mg lysate
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RNF5
Long Name
Ring Finger Protein 5
Alternate Names
Protein G16, Ram1 Homolog, RING5, RMA1
Gene Symbol
RNF5
UniProt
Additional RNF5 Products
Product Documents for RNF5 Antibody - BSA Free
Product Specific Notices for RNF5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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