RNF5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94913

Novus Biologicals, part of Bio-Techne
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NBP2-94913-0.02ml
NBP2-94913-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RNF5 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RNF5 (NP_008844.1). MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNF5 Antibody - BSA Free

Western Blot: RNF5 AntibodyBSA Free [NBP2-94913]

Western Blot: RNF5 AntibodyBSA Free [NBP2-94913]

Western Blot: RNF5 Antibody [NBP2-94913] - Analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
Immunohistochemistry-Paraffin: RNF5 Antibody - BSA Free [NBP2-94913]

Immunohistochemistry-Paraffin: RNF5 Antibody - BSA Free [NBP2-94913]

Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Rat liver using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: RNF5 Antibody - BSA Free [NBP2-94913]

Immunohistochemistry-Paraffin: RNF5 Antibody - BSA Free [NBP2-94913]

Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Human liver cancer using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for RNF5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF5

The protein encoded by the RNF5 gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. (provided by RefSeq)

Long Name

Ring Finger Protein 5

Alternate Names

Protein G16, Ram1 Homolog, RING5, RMA1

Gene Symbol

RNF5

Additional RNF5 Products

Product Documents for RNF5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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