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RNF6 Antibody (3B1)

Novus Biologicals, part of Bio-Techne | Catalog # H00006049-M01

Novus Biologicals, part of Bio-Techne
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H00006049-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RNF6 (NP_005968, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSEELQQRLDGVKEQLASQPDLRDGTNYRDSEV

Specificity

RNF6 - ring finger protein (C3H2C3 type) 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RNF6 Antibody (3B1)

Immunocytochemistry/ Immunofluorescence: RNF6 Antibody (3B1) [H00006049-M01]

Immunocytochemistry/ Immunofluorescence: RNF6 Antibody (3B1) [H00006049-M01]

Immunocytochemistry/Immunofluorescence: RNF6 Antibody (3B1) [H00006049-M01] - Analysis of monoclonal antibody to RNF6 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: RNF6 Antibody (3B1) [H00006049-M01] - Detection limit for recombinant GST tagged RNF6 is approximately 0.03ng/ml as a capture antibody.

Applications for RNF6 Antibody (3B1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF6

The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed.

Alternate Names

DKFZp686P0776, E3 ubiquitin-protein ligase RNF6, EC 6.3.2.-, ring finger protein (C3H2C3 type) 6, RING-H2 protein RNF-6, SPG2

Entrez Gene IDs

6049 (Human)

Gene Symbol

RNF6

OMIM

133 (Human)

Additional RNF6 Products

Product Documents for RNF6 Antibody (3B1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF6 Antibody (3B1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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