RNF7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85594

Novus Biologicals, part of Bio-Techne
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NBP1-85594
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Summary for RNF7 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF7 Antibody

Western Blot: RNF7 Antibody [NBP1-85594]

Western Blot: RNF7 Antibody [NBP1-85594]

Western Blot: RNF7 Antibody [NBP1-85594] - Analysis in control (vector only transfected HEK293T lysate) and RNF7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RNF7 Antibody [NBP1-85594]

Immunocytochemistry/ Immunofluorescence: RNF7 Antibody [NBP1-85594]

Immunocytochemistry/Immunofluorescence: RNF7 Antibody [NBP1-85594] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
RNF7 Antibody

Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-85594] -

Immunohistochemistry-Paraffin: RNF7 Antibody [NBP1-85594] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells.
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Applications for RNF7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF7

RNF7 is encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

CKBBP1zinc RING finger protein SAG, CKII beta-binding protein 1, Rbx2, Regulator of cullins 2, ring finger protein 7rbx2, ROC2RING-box protein 2, SAGsensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2, Sensitive to apoptosis gene protein

Gene Symbol

RNF7

Additional RNF7 Products

Product Documents for RNF7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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