RNF8 Antibody (JE56-43)

Western Blot: RNF8 Antibody (JE56-43) [NBP3-32912]

Western Blot: RNF8 Antibody (JE56-43) [NBP3-32912] - Western blot analysis of RNF8 on human brain tissue lysates with Rabbit anti-RNF8 antibody (NBP3-32912) at 1/2,000 dilution.

Lysates/proteins at 40 ug/Lane.

Predicted band size: 56 kDa
Observed band size: 63 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32912) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RNF8 Antibody (JE56-43) [NBP3-32912]

Immunohistochemistry: RNF8 Antibody (JE56-43) [NBP3-32912] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-RNF8 antibody (NBP3-32912) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32912) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.